if you can buy scsi only. lol, rip off, but is much better then ata. i have a pc, Athlon XP 1700+ 1.47ghz. 512mb ddr ram. ata/100 controller on mobo and 7200rpm drives. 80gb and 40gb. on different channels too. when copying a large folder from one drove to the other it slows down my pc to a crawl at times. wish SCSI this would be noticably faster so then my pc wouldn't slow down as much. but then scsi is 3-5 times the cost. or more.
Like for a U160 scsi card $300 or little less. $200-$275 for 18-24gb scsi drive. U160 one.
Anyhow if you have ata/66 and go to ata/100 you won't notice much difference at all. I went from ata/33 to ata/100 controller on a hard drive a while ago and noticed roughly 40% increase in speed. like 40% faster to copy same folder. this on older computer, not my current one. so with you it be a lot less increase in speed. going from 5400 to 7200 will help a bit, but not huge. you'll see it i think.
if you can go scsi, if not then get 7200 rpm and no need for ata/100 pci card, but as they are only $40-$50 then I'd get one. well promise tech ata/100 for pc's cost $40-$50.