Glad i read this post. i just downloaded the iDefrag Demo. I am a Storage Engineer who mainly does large Unix systems - wish I had a tool like iDefrag.
So, quick answer; all disks (filesystems actually) get fragmented. If you logically model your disk as a 2-D circle viewed from the top, the outer portion of the disk (tracks) have the lower-numbered addresses & get used first by the O/S Filesystem. Compared to the inner tracks, data access is about 25% faster on the outer versus inner tracks. If you delete some files on these outer tracks, this coveted disk real estate will get used to create new files.
I have a 4 year old Mac mini that is 41% full. iDefrag said 527 out of 372K files are fragmented(.1%; not much). About 50% of my fragmented files have 3 or less fragments. The top 3 fragmented files at 672, 440, & 416 fragments (pretty darn fragmented in my opinion) and are the Safari SafeBrowsing.db files for the 3 accounts on the system.
If you are concerned about issues such as this you should consider a disk defrag tool.
Be careful. The doc's for iDefrag caution that you should backup your computer before using.
If you are serious about your data you should place your applications ie the /Applications directory and your data ie the /Users directory on separate filesystems & only defrag the data. That way if defrag breaks your filesystem, you only break /Users & you still have an O/S for recovery instead of installing/configuring the whole system from scratch.